γ-Glutamyl Valine, Found in Dry Edible Beans, Is Anti-diabetic in db/db Mice
نویسندگان
چکیده
Dietary γ-glutamyl peptides (γ-GPs) found in dry edible beans exhibit biological activities (antioxidant, anti-inflammatory) with potential benefits against chronic metabolic disorders. γ-GPs are responsible for the desirable Kokumi flavor through allosteric activation of Calcium Sensing Receptor (CaSR) present multiple mammalian tissues. In this study, we investigated effects valine (γ-EV) diabetic obese mice. Four-weeks old male db/db mice (BKS.Cg-Dock7m +/+ Leprdb/J) were fed AIN-93G diet ad libitum and given water or without γ-EV (500 mg/kg body weight) 3 weeks. After weeks intervention, blood, intestine liver collected to determine blood glucose, plasma concentrations (LC-MS/MS), alanine aminotransferase (ALT) activity, jejunum transcriptomes (RNA-Seq), protein expression (Western blot), glycogen content. Mice had higher weight gain (45%, p < 0.001, n = 8), lower food intake (21%, 0.0001), better efficiency (79%, 0.0001) than their control counterparts. reached 2.07 ± 0.56 μM. Blood glucose levels decreased (29%, 0.01) urination was markedly improved. The hepatosomatic index increased (66%, 0.0001); however, ALT activity not significantly changed. RNA-Seq analysis revealed 147 jejunal genes 1308 differentially expressed due intake, 26 these common top GO categories affected by fatty acid process (17/193 genes), lipid (22/545 peroxisome (11/136 genes); liver, oxidoreductase (104/686 (79/545 iron ion binding (43/185 genes). Hepatic AMP-activated kinase (p-AMPKα, Thr172) abundance, a major cellular regulator metabolism, (86%, 0.05), treatment group suggesting induced catabolism. improved condition via modulation metabolism. Funding provided University Nebraska-Lincoln Collaboration Initiative Nebraska Dry Bean Commission.
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ژورنال
عنوان ژورنال: Current developments in nutrition
سال: 2022
ISSN: ['2475-2991']
DOI: https://doi.org/10.1093/cdn/nzac053.080